Ultrathin polyethyleneimine (PEI) films for culturing of the human mesenchymal stromal cells (hMSCs)
Main Article Content
Abstract
A polymer polyethyleneimine (PEI) can be used in various cell technologies, including the nonviral transfection of animal and human eukaryotic cells. We used the polymer (in the 1 mg/ml concentration) to produce the ultrathin films at the culture plastic that could enhance the adhesive properties of the human mesenchymal stromal cells (hMSCs) isolated from the heterogeneous cell population of the marrow. Our study demonstrated that the PEI films prevent cell-to-cell cooperation and “glomeration” of hMSCs at the surface of a cultural plate, and enhance hMSCs viability and metabolic activity in vitro. The stimulating effects of PEI on cell proliferation are negligible. Moreover, the immobilization of the human platelet lysate (PL) in 5% or 10% concentrations into the PEI solution increases hMSCs viability and effectiveness of their culturing under the deprivation condition and in the serum-free medium. We suggest to use the PEI films containing the immobilized growth factors in the regenerative medicine – at the stages of hMSCs isolation and growth of their biomass.
Downloads
Article Details
Copyright (c) 2020 Mezhevikina LM, et al.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing and protecting the author rights is the central aim and core of the publishing business. Peertechz dedicates itself in making it easier for people to share and build upon the work of others while maintaining consistency with the rules of copyright. Peertechz licensing terms are formulated to facilitate reuse of the manuscripts published in journals to take maximum advantage of Open Access publication and for the purpose of disseminating knowledge.
We support 'libre' open access, which defines Open Access in true terms as free of charge online access along with usage rights. The usage rights are granted through the use of specific Creative Commons license.
Peertechz accomplice with- [CC BY 4.0]
Explanation
'CC' stands for Creative Commons license. 'BY' symbolizes that users have provided attribution to the creator that the published manuscripts can be used or shared. This license allows for redistribution, commercial and non-commercial, as long as it is passed along unchanged and in whole, with credit to the author.
Please take in notification that Creative Commons user licenses are non-revocable. We recommend authors to check if their funding body requires a specific license.
With this license, the authors are allowed that after publishing with Peertechz, they can share their research by posting a free draft copy of their article to any repository or website.
'CC BY' license observance:
|
License Name |
Permission to read and download |
Permission to display in a repository |
Permission to translate |
Commercial uses of manuscript |
|
CC BY 4.0 |
Yes |
Yes |
Yes |
Yes |
The authors please note that Creative Commons license is focused on making creative works available for discovery and reuse. Creative Commons licenses provide an alternative to standard copyrights, allowing authors to specify ways that their works can be used without having to grant permission for each individual request. Others who want to reserve all of their rights under copyright law should not use CC licenses.
Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, et al. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A 92: 7297–7301. Link: https://bit.ly/2Y7YH6T
Guerra-Crespo M, Charli JL, Rosales-Garcia VH, Pedraza-Alva G, Perez-Martinez L (2003) Polyethylenimine improves the transfection efficiency of primary cultures of post-mitotic rat fetal hypothalamic neurons. J Neurosci Methods 127: 179-192. Link: https://bit.ly/3kWx7TV
Neu M, Fischer D, Kissel T (2005) Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives. J Gene Med 7: 992-1009. Link: https://bit.ly/34mN5kL
Gabrielson NP, Pack DW (2006) Acetylation of polyethylenimine enhances gene delivery via weakened polymer/DNA Interactions. Biomacromolecules 7: 2427-2435. Link: https://bit.ly/2CFjuaE
Pandey AP, Sawant KK (2016) Polyethylenimine: A versatile, multifunctional non-viral vector for nucleic acid deliver. Mat Sci Engin C 68: 904-918. Link: https://bit.ly/2E1HHZm
Futami J, Kitazoe M, Maeda T, Nukui E, Sakaguchi M, et al. (2005) Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization. J Biosci Bioeng 99: 95-103. Link: https://bit.ly/2E7BOtj
Kitazoe M, Murata H, Futami J, Maeda T, Sakaguchi M, et al. (2005) Protein transduction assisted by polyethylenimine-cationized carrier proteins. J Biochem 137: 693-701. Link: https://bit.ly/2E2ktSW
Kitazoe M, Futami J, Nishikawa M, Yamada H, Maeda Y (2010) Polyethylenimine-cationized β-catenin protein transduction activates the Wnt canonical signaling pathway more effectively than cationic lipid-based transduction. Biotechnol J 5: 385-392. Link: https://bit.ly/3h7Cf5z
Remy-Kristensen A, Clamme JP, Vuilleumier C, Kuhry JG, Mely Y (2001) Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes. Biochim Biophys Acta 1514: 21–32. Link: https://bit.ly/2CBXi17
Di Gioia S, Rejman J, Carrabino S, De Fino I, Rudolph C, et al. (2008) Role of Biophysical Parameters on ex Vivo and in Vivo Gene Transfer to the Airway Epithelium by Polyethylenimine/Albumin Complexes. Biomacromol 9: 859–866. Link: https://bit.ly/2CAtK3N
Akinc A, Mini T, Klibanov AM, Langer R (2005) Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis. J Gene Med 7: 657–663. Link: https://bit.ly/2CAtMZt
Shen C, Li J, Zhang Y, Li Y, Shen G, et al. (2017) Polyethylenimine-based micro/nanoparticles as vaccine adjuvants. Int J Nanomed 12: 5443–5460. Link: https://bit.ly/3g76hoH
Milovic NM, Wang J, Lewis K, Klibanov AM (2005) Immobilized N-alkylated polyethylenimine avidly kills bacteria by rupturing cell membranes with no resistance developed. Biotechnol Bioeng 90: 715-722. Link: https://bit.ly/3iOxHkW
Khalil H, Chen T, Riffon R, Wang R, Wang Z (2008) Synergy between polyethylenimine and different families of antibiotics against a resistant clinical isolate of Pseudomonas aeruginosa. Antimicrob Agents Chemother 52: 1635–1641. Link: https://bit.ly/3h85zZI
Spoden GA, Besold K, Krauter S, Plachter B, Hanik N, et al. (2012) Polyethylenimine is a strong inhibitor of human papillomavirus and cytomegalovirus infection. Antimicrobial Agents and Chemotherapy 56: 75–82. Link: https://bit.ly/3g3DgKF
Liu J, Su D, Yao J, Huang Y, Shao Z, et al. (2017) Soy protein-based polyethylenimine hydrogel and its high selectivity for copper ions removal in wastewater treatment. J Mater Chem A 4: 1-3. Link: https://rsc.li/2CArhq4
Vancha AR, Govindaraju S, Parsa KVL, Jasti M, González-García V, et al. (2004) Use of polyethyleneimine polymer in cell culture as attachmen. BMC Biotechnol 4: 23. Link: https://bit.ly/2FAix4w
Ai H, Lvov YM, Mills DK, Jennings M, Alexander JS, et al. (2003) Coating and selective deposition of nanofilm on silicone rubber for cell adhesion and growth. Cell Biochem Biophys 38: 103-114. Link: https://bit.ly/3kWjeFy
Ruardij TG, Goedbloed MH, RuttenWLC (2000) Adhesion and patterning of cortical neurons on polyethylenimine- and fluorocarbon-coated surfaces. Trans Biomed Engineering 47: 1593-1599. Link: https://bit.ly/3h5KcrO
Liu B, Ma J, Gao E, He Y, Cui F, et al. (2008) Development of an artificial neuronal network with post-mitotic rat fetal hippocampal cells by polyethylenimine. Biosens Bioelect 23: 1221–1228. Link: https://bit.ly/2DUSF2Z
Lelong IH, Petegnief V, Rebel G (1992) Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates. J Neurosci Res 32: 562-568. Link: https://bit.ly/317EZu5
Bledi Y, Domb AJ, Linial M (2000) Culturing neuronal cells on surfaces coated by a novel polyethyleneimine-based polymer. Brain Res 5: 282-289. Link: https://bit.ly/321m3wj
Garza JM, Jessel N, Ladam G, Dupray V, Muller S, et al. (2005) Polyelectrolyte multilayers and degradable polymer layers as multicomponent films. Langmuir 21: 12372-12377. Link: https://bit.ly/3g4BXLu
Nadri S, Soleimani M, Hosseni RH, Massumi M, Atashi A, et al. (2007) An efficient method for isolation of murine bone marrow mesenchymal stem cells. Int J Dev Biol 51: 723-729. Link: https://bit.ly/316N1mR
Rivkin R, Ben-Ari A, Kassis I, Zangi L, Gaberman E, et al. (2007) High-yield isolation, expansion, and differentiation of murine bone marrow-derived mesenchymal stem cells using fibrin microbeads (FMB). Cloning Stem Cells 9: 157-175. Link: https://bit.ly/324K7ya
Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147. Link: https://bit.ly/34mP4pd
Parhamifar L, Larsen AK, Hunter AC, Andresen TL, Moghimi SM (2010) Polycation cytotoxicity: a delicate matter for nucleic acid therapy focus on polyethylenimine. Soft Matter 6: 4001-4009. Link: https://rsc.li/2E64TWe
Kafil V, Omidi Y (2005) Cytotoxic impacts of linear and branched polyethylenimine nanostructures in A431 cells. BioImpacts 2011; 1: 23-30. Link: https://bit.ly/3iTEb2a
Moghimi SM, Symonds P, Murray JC, Hunter AC, Debska G, et al. (2005) A tow-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene-transfer/therapy. Mol Ther 11: 900-995. Link: https://bit.ly/2Frr6hQ
Hunter AC, Moghimi SM (2010) Cationic carriers of genetic material and cell death: a mitochondrial tale. Biochim Biophys Acta Bioenerg 1797: 1203-1209. Link: https://bit.ly/2E9Cbnl
Oku N, Yamaguchi N, Yamaguchi N, Shibamoto S, Ito F, et al. (1986) The fusogenic effect of synthetic polycations on negatively charged lipid bilayers. J Biochem 100: 935–944. Link: https://bit.ly/2DTiVuz
Boeckle S, von Gersdorff K, van der Piepen S, Culmsee C, Wagner E, et al. (2004) Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer. J Gene Med 6: 1102-1111. Link: https://bit.ly/315VpmH
Zhang C, Wu Fu-Gen, Hu P, Chen Z (2014) Interaction of polyethylenimine with model cell membranes studied by linear and nonlinear spectroscopic techniques. J Phys Chem C 118: 12195-12205. Link: https://bit.ly/3iNgXKX
Ira, Mély Y, Krishnamoorthy G (2003) DNA vector polyethyleneimine affects cell pH and membrane potential: a time-resolved fluorescence microscopy study. J Fluorescence 13: 339-347. Link: https://bit.ly/2YeA34S
Grzeczkowicz A, Gruszczynska-Biegala J, Czeredys M, Kwiatkowska A, Strawski M, et al. (2019) Polyelectrolyte membrane scaffold sustains growth of neuronal cells. J Biomed Mater Res Part A 107: 839-850. Link: https://bit.ly/3g7Gflc
Clamme JP, Bernacchi C, Vuilleumier C, Duportail G, Mely Y (2000) Gene transfer by cationic surfactants is essentially limited by the trapping of the surfactant/DNA complexes onto the cell membrane: a fluorescence investigation. Biochim Biophys Acta 1467: 347–361. Link: https://bit.ly/312YmEC